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Image Search Results
Journal: bioRxiv
Article Title: Parabrachial neuron types categorically encode thermoregulation variables during heat defense
doi: 10.1101/2020.06.11.138370
Figure Lengend Snippet: a-c , Changes in basal T core ( a ), basal physical activity ( b ), and basal energy expenditure ( c ) after blocking of POA-projected LPB Pdyn neurons (n = 6 each; 4-6 weeks post viral injection). d-g , Changes of energy expenditure ( d-e ), and physical activity ( f-g ) during warm challenge ( d, f ) or cold challenge ( e, g ), respectively, after blocking of POA-projected LPB Pdyn neurons (n = 6 each; 6-8 weeks post viral injection). (– 360 – 0 min) and (0 – 360 min) represent the averaged EE between t = (– 360 – 0 min) and t = (0 – 360 min), respectively. All data are shown as mean ± s.e.m. All the p-values are calculated based on ordinary two-way ANOVA with Bonferroni’s corrections (right panels in a-g ). *p ≤ 0.05; ns, not significant.
Article Snippet: Primary antibodies include chicken anti-GFP (Abcam, #ab13970, 1: 1000), rabbit anti-Neuropeptide S (Abcam, #ab18252, 1: 500), rabbit anti-cfos (Synaptic systems, #226003, 1: 10000), guinea pig anti-cfos (Synaptic systems, #226004, 1: 500), rat anti-RFP (Chromotek, #5F8, 1: 1000), rabbit anti-Glutamate (Sigma, #G6642-.2ML, 1: 1000; see ( - ) for validation.), rabbit anti-Dynorphin A (Phoenix Pharmaceuticals, #H-021-03, 1:500) and
Techniques: Activity Assay, Blocking Assay, Injection